應(yīng)用編號(Application ID):
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):離子交換(Ion-exchange chromatography)
產(chǎn)品系列(Product Line):Antibodix
方法(Method):
| 
             Column:  | 
            
             Antibodix WCX-NP10, 10 µm, 4.6 x 250 mm  | 
        
| 
             Mobile phase:  | 
            
             A: 10 mM Sodium phosphate buffer, pH 7.5 B: A + 0.1 M NaCl  | 
        
| Time( min ): | 0 30 35 40 41 50 51 80 | 
| A%: | 90 55 35 35 0 0 90 90 | 
| B%: | 10 45 65 65 100 100 10 10 | 
| 
             Flow rate:  | 
            
             0.8 mL/min  | 
        
| 
             Temperature:  | 
            
             30 oC  | 
        
| 
             Detection:  | 
            
             UV 214 nm  | 
        
| 
             Injection volume:  | 
            
             50 µL  | 
        
| 
             Sample:  | 
            
             1.0 mg/mL antibody  | 
        
