應(yīng)用編號(hào)(Application ID):
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):離子交換(Ion-exchange chromatography)
產(chǎn)品系列(Product Line):Antibodix
方法(Method):
Column: |
Antibodix WCX-NP5, 5 µm, 4.6 x 250 mm |
Mobile phase: |
A: 20 mM HEPES, pH 7.5 B: A + 0.1 M NaCl |
Time( min ): | 0 30 40 |
A%: | 100 94 90 |
B%: | 0 6 10 |
Flow rate: |
0.6 mL/min |
Temperature: |
30 oC |
Detection: |
UV 280 nm |
Injection volume: |
10 µL |
Sample: |
2.0 mg/mL antibody |