應(yīng)用編號(hào)(Application ID):
樣品分類(Sample Category):生物化學(xué)(Biochemistry)
分離模式(Separation Mode):離子交換(Ion-exchange chromatography)
產(chǎn)品系列(Product Line):Antibodix
方法(Method):
| 
             Column:  | 
            
             Antibodix® WCX-NP5 ( 5 µm, 4.6 x 250 mm )  | 
        
| 
             Mobile phase:  | 
            
             A: 4 mM Tris, 4 mM imidazole, 4 mM piperazine( pH 8.0 ), B: A at pH 10.83  | 
        
| 
             Flow rate:  | 
            
             0.8 mL/min  | 
        
| 
             Temperature:  | 
            
             25℃  | 
        
| 
             Detection:  | 
            
             UV 280 nm  | 
        
| 
             Injection volume:  | 
            
             10 µL  | 
        
| 
             Sample:  | 
            
             about 5 mg/mL IgG2  | 
        
